Gliadins are mostly monomeric proteins with molecular weights between 28,000 and 55,000 and can be classified, depending on their different primary structures, into a/b, y and o types. They can be found in wheat and several other cereals of the genus Triticum (Wieser, 1996). For each type, the structural differences between them are small. This is due to the substitution, deletion and insertion of individual amino acid residues (Weiser, 2007). These proteins contain unusually high amounts of glutamine and proline and have large regions of repetitive sequences. Because most gliadins are monomeric proteins that contain eight conserved cysteine ​​residues, some contain an extra cysteine ​​residue that allows them to be linked to other gluten proteins to form large polymers essential for flour quality ( Altenbach et al, 2010). ELISA can be used to detect the presence of antibodies and forms the basis of testing for human immunodeficiency virus (HIV) (Berg et al, 2002). In this test, viral core proteins (the antigen) are absorbed to the bottom of a well. Antibodies collected from the patient are then added to the coated well to bind to the antigen. Finally, enzyme-bound antibodies and human antibodies, such as goat antibodies that recognize human antibodies, react within the well, and any unbound antibodies are removed by washing the plate. The substrate is then applied to the well. If an enzymatic reaction occurs, this suggests that the antibodies bound to the enzyme were bound to human antibodies, which in turn implies that the patient had antibodies to the viral antigen (Lennette et al, 1987). A disadvantage of the single antibody system is its reduced sensitivity since the signal is not ... middle of the paper ... and formation as it can contribute to both hemostasis and thrombosis. Platelet formation may also contribute to inflammation including immune-mediated inflammation and the development of atherosclerosis (Yang et al, 2009). This makes measurement of fibrinogen important for these diseases. Conclusion To conclude, the ELISA method is effective in detecting various diseases and is also an ideal method for screening for diseases and toxins. There is also a wide variety of ELISA methods that can be used depending on the antibody and antigens that need to be detected. Additionally, as mentioned, they are highly sensitive, meaning they can provide accurate and reliable results. They are also relatively cheap and do not require a high level of skill or expensive equipment to use, but can be subject to experimental errors if procedures are not followed.