The microbial fuel cell (MFC) is an emerging technology in the field of bioenergy generation along with wastewater treatment. Microbial fuel cell generates energy with the help of microbes making it a future green energy source. In MFC, anaerobic microbes degrade organic matter and produce hydrogen ions (H+) and electrons (e-) at the anode. The H+ ion diffuses across the proton exchange membrane (PEM); and e- are transported through an electrode via a circuit external to the cathode. At the cathode, e- and H+ ions combine with oxygen to form water (H2O), which results in energy generation [1]. MFC has an open circuit voltage (OCV) limited to 1.14 V due to the standard redox potential of NADH/NAD+ and O2/H2O of -0.32 V and +0.82 V, respectively [2]. NAD+ (Nicotinamide Adenine Dinucleotide) is a coenzyme present in all living cells. It is involved in redox reactions, transporting electrons into the reaction during metabolism. Dual-chamber MFC has been proven to be more efficient than single-chamber MFC for treatment efficiency such as removal of COD, BOD, nitrates, etc. [3, 4]. The advantage of aerobic conditions at the cathode can be obtained in both abiotic and biotic forms. Recently, the biocathode has attracted the attention of many researchers due to the increase in efficiency. In a biocathode, microbes act as electronic mediators and also provide aerobic treatment. It also provides a variety of terminal electron acceptors, and microbes catalyze the electron transfer, decreasing the need for expensive metals such as platinum and electrolyte [6]. Sole et al. [7] achieved a 300% increase in power density using the biocathode. Therefore, sequentially configured bio-anode (anaerobic treatment) and bio-cathode (aerobic treatment) assemblies can improve paper flow using stacked microbial fuel cells. Environment. Sci. Technology. 2006; 39: 3388–3394.[19] Ieropoulos I, Greenman J, Melhuish C. Microbial fuel cell based on carbon film electrodes: stack configuration and scalability. internal J.Energy resource. 2008; 32: 1228–1240.[ 20] Timmers RA, Strik DPBTB, Hamelers HVM, Buisman CJN. Electricity generation using an innovatively designed tubular plant-based microbial fuel cell. biomass bioenergy 2013; 51: 60-67.[21] Gálvez A, Greenman J, Ieropoulos I. Treatment of landfill leachate with microbial fuel cells; grow through plurality. Bioresource. Technology. 2009; 100: 5085–5091.[22] Wang B, Han JI. A single-chamber stackable microbial fuel cell with an air cathode. Biotechnology. Letters 2009; 31:387–393.[23] Jia B, Hu D, Xie B, Dong K, Liu H. Increasing power density from a spiral microbial fuel cell. Bioelectron biosensors. 2013; 41: 894–897.